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通过准miR-544/WDR12轴,circMIRIAF通过准miR-544/WDR12轴加剧心肌缺血-再输液损伤

Lianhong Yin¹, Lili Li², Meng Gao¹

¹Department of Pharmaceutical Analysis, Dalian Medical University, Western 9 Lvshunnan Road, Dalian, 116044, China.

|May 25, 2024

概括

循环RNAs (circRNAs) 为心肌缺血-再输液损伤 (MI/RI) 提供了新的药物标. CircMIRIAF通过菌miR-544,增加WDR12表达和激活Notch1信号来加剧MI/RI.

科学领域:

心血管生物学心血管生物学
分子医学是分子医学.
生物化学生物化学

背景情况:

心肌缺血-反损伤 (MI/RI) 带来了重大的临床挑战.
迫切需要针对MI/RI的新型治疗点.
循环RNAs (circRNAs) 正在成为心血管疾病的关键调节者.

研究的目的:

为了研究circRNAs在心肌缺血-反损伤 (MI/RI) 中的作用.
为了确定特定的circRNAs涉及到心肌细胞的低氧-重氧化损伤 (H/RI).
阐明circMIRIAF在调节MI/RI中的分子机制.

主要方法:

微阵列查以识别AC16细胞中的调节失调的circRNAs.
生物信息分析用于构建circRNA-miRNA-mRNA ceRNA网络.
在体外和体内实验验验证circMIRIAF,miR-544和WDR12的功能.

主要成果:

鉴定出hsa_circRNA_104852 (circMIRIAF) 在低氧-重氧化损伤 (H/RI) 中具有上调调.
circMIRIAF 作为 miR-544 的海绵,导致 WDR12 蛋白质表达的增加.
通过促进氧化应激和炎症,WDR12激活了Notch1信号,加剧了H/RI和MI/RI.

结论:

circMIRIAF可以作为MI/RI的潜在治疗点.
环MIRIAF/miR-544/WDR12轴在加重MI/RI方面发挥着至关重要的作用.
向circMIRIAF可能为MI/RI治疗提供一种新的治疗策略.

关键词:

炎症炎症是一种炎症.心肌缺血-再输血损伤的心肌缺血.氧化应激是一种氧化应激.这就是WDR12的WDR12.这是一个圆周.在 miR-54444 的位置上.

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