在circ-EIF4A3和EIF4A3之间的调控反循环 增强肠直肠癌细胞的自和生长
Qingke Li1, Zhiwu Wang2, Jian Wang1
1Department of Gastrointestinal Surgery, Tangshan People's Hospital, Tangshan 063000, Hebei, China.
Translational oncology
|May 25, 2024
概括
循环RNAs (circRNAs) 和真核转化启动因子4A3 (EIF4A3) 促进结直肠癌 (CRC) 的生长. 涉及circEIF4A3和EIF4A3的反循环可以增强CRC细胞自和增殖.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 循环RNAs (circRNAs) 在结直肠癌 (CRC) 进展中起着重要作用.
- 细胞转化启动因子4A3 (EIF4A3) 参与促进circRNA生物发生.
- 在CRC细胞自中,EIF4A3衍生的circRNA (circEIF4A3) 的特定功能尚不清楚.
研究的目的:
- 为了研究circEIF4A3对结直肠癌细胞生长和自的影响.
- 阐明circEIF4A3在CRC中的作用背后的分子机制.
主要方法:
- 研究了EIF4A3和circEIF4A3对CRC细胞增殖的协同作用.
- 分析了circEIF4A3,miR-3126-5p和EIF4A3表达之间的相互作用.
- 通过circEIF4A3和EIF4A3.3.3检查了与自相关的基因 (ATG5,ATG7) 和二化酶 (USP14) 的调节.
- 评估了CRC细胞中EIF4A3和circEIF4A3形成之间的反机制.
主要成果:
- 发现EIF4A3和circEIF4A3可以协同促进CRC细胞生长.
- 证明CircEIF4A3可以封存miR-3126-5p,从而导致EIF4A3表达的增加.
- CircEIF4A3增强了EIF4A3的表达,通过稳定ATG5mRNA促进了自,并通过USP14mRNA稳定增强了ATG7蛋白的稳定性.
- 提高ATG5和ATG7表达的调节抵消了EIF4A3淘汰的增长抑制作用.
- 已经证明EIF4A3可以诱导CRC细胞中的circEIF4A3形成.
结论:
- 在结直肠癌中,circEIF4A3和EIF4A3之间存在正反循环.
- 这种反循环通过增强细胞自来支持CRC细胞生长.
- CircEIF4A3和EIF4A3代表了结直肠癌治疗的潜在治疗点.
相关概念视频
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:22PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:19Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
01:15Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
02:38Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K