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在多孔聚合物上优化固定尿酶酶以提高稳定性,可重复使用性和酶动力学,使用响应表面方法

Busra Sahin¹, Bahar Ozbey-Unal², Nadir Dizge³

¹Department of Biotechnology, Gebze Technical University, Gebze 41400, Turkey.

Colloids and surfaces. B, Biointerfaces

|May 25, 2024

概括

这项研究优化了polyHIPE材料上的尿酶固定,达到95%的效率. 固定性尿酶显示出出色的可重复使用性和稳定性,为生物催化剂应用提供了潜力.

科学领域:

生物技术是生物技术.
酶不移动化酶不移动化
材料科学是一种材料科学.

背景情况:

尿酶在各种工业过程中至关重要.
有效的固定是提高酶稳定性和可重复使用性的关键.
高内部相乳液 (polyHIPE) 材料为酶固定提供了一个有希望的支架.

研究的目的:

为了研究尿酶对多HIPE材料的固定.
通过响应表面方法来优化固定条件.
评估固定尿酶的特性,效率和性能.

主要方法:

扫描电子显微镜 (SEM) 和富里埃变换红外光谱 (FT-IR) 用于材料表征.
响应表面方法 (RSM) 的Box-Behnken设计 (BBD) 进行优化.
酶活性测试以确定固定效率,可重复使用性和稳定性.

主要成果:

通过多聚甲 (PGA) 修饰和15小时化,达到95%的最大固定效率.
优化条件:40.8°C,pH7.1和0.007g/L NaCl. 这就是最佳条件.
固定尿酶在六个周期后保持了75%的活性,在10天后保持了> 40%.
动力分析显示基质亲和度较高,但固定尿酶的转化率较低.

结论:

聚HIPE材料是尿酶固定化的有效支持.
优化的固定条件显著提高了酶的性能.
固定性尿酶具有很高的可重复使用性和稳定性,适合工业应用.
这项工作为开发强大的生物催化剂提供了基础.

关键词:

葡萄糖甲化物葡萄糖甲化物固定化固定化固定化多种类型的HIPE.尿素去除尿素的方法在Urease中使用Urease.

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