TJP1通过在人类胎盘中表达E2F8来抑制热囊细胞的入侵
Rika Miki1, Seiko Matsuo2, Takafumi Ushida3
1Laboratory of Bell Research Center Department of Obstetrics and Gynecology Collaborative Research, Nagoya University Graduate School of Medicine, Nagoya, Aichi, 466-8550, Japan.
Molecular and cellular endocrinology
|May 25, 2024
概括
E2F8转录因子通过促进TJP1表达来抑制热囊细胞的入侵. 在子前期增加的TJP1表明在胎盘发育和疾病发病过程中发挥了作用.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 分子遗传学 分子遗传学
背景情况:
- 外热囊细胞 (EVT) 侵袭对人类胎盘发育至关重要.
- 失调的EVT入侵与妊娠并发症,如子宫前有关.
- 调节EVT入侵的分子机制需要进一步阐明.
研究的目的:
- 研究E2F转录因子8 (E2F8) 和紧结蛋白-1 (TJP1) 在人类胎盘发育中的作用.
- 为了确定E2F8和TJP1之间的监管关系,表达.
- 探索E2F8和TJP1在孕前病原发生的潜在参与.
主要方法:
- 研究了E2F8和TJP1表达在人类胎盘和外性细胞中.
- 使用的细胞培养模型 (HTR-8/SVneo细胞) 使用E2F8敲击和TJP1操纵.
- 在E2F8过度表达后评估TJP1转录和蛋白质水平.
主要成果:
- 在HTR-8/SVneo细胞中,E2F8敲击降低了TJP1表达.
- 在整个妊娠期内,TJP1和E2F8在胎盘小和EVT中共同表达.
- 与对照组相比,TJP1水平在前胎盘中升高.
- 增强了TJP1的淘汰作用,同时抑制了TJP1的过度表达,对热囊细胞的侵入.
- E2F8过度表达上调了TJP1的表达和转录.
结论:
- E2F8促进了TJP1的转录,而TJP1抑制了EVT的入侵.
- E2F8和TJP1与调节胎盘发育有关.
- 异常的E2F8和TJP1表达可能有助于孕前的发病.
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