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分段病毒的核酸和二核酸偏好更多地依据分段形成:在番茄斑点枯病毒的案例研究中

Haiting Zhao¹, Lang Qin¹, Xiaolong Deng¹

¹College of Plant Protection, Yangzhou University, Yangzhou 225009, China.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

|May 25, 2024

概括

病毒核酸组成主要是由病毒细分和蛋白质编码区域决定的,而不是宿主物种. 这一发现为细分病毒的分子进化提供了新的见解,例如番茄斑点变病毒 (TSWV).

科学领域:

病毒学病毒学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

病毒核酸和二核酸组成可能与宿主物种或蛋白质编码区域相关.
病毒细分对这些组成模式的影响在很大程度上仍未被探索.

研究的目的:

研究病毒片段对细分病毒中核酸和二核酸组成的影响.
分析番茄斑点枯病毒 (TSWV) 作为一个模型系统.

主要方法:

跨不同病毒段和蛋白质编码区域的核酸组成分析.
在完整的基因组,细分,编码区域和宿主物种中进行二核酸频率分析.
预测建模以评估基于序列组成的细分和主机预测的准确性.

主要成果:

在所有TSWV段中,在第一个和第二个编码子位置上,氨酸 (A) 的持续过度代表性.
在第三个编码位和蛋白质编码区域之间观察到明显的核酸偏好.
特定的二核酸模式 (UpG和CpU的过度代表;UpA,CpG和GpU的不足代表) 在各个细分和编码区域中得到保留.
序列组成准确地预测了病毒细分 (100%) 和蛋白质编码区域 (100%),但对宿主物种 (67%) 的预测较低.

结论:

病毒段和蛋白质编码区域是核酸和二核酸组成的更强的决定因素,而不是TSWV的宿主物种.
这些发现为TSWV分子进化和细分病毒的遗传多样性提供了新的视角.

关键词:

丁核酸的偏好丁核酸的偏好主体物种是宿主物种.蛋白质编码区域是蛋白质的编码区域.分段式病毒分段式病毒.番茄斑点枯病毒病毒

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