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从废水样本中检测和量化SARS-CoV-2RNA的不同分子系统的评估

Fabio Morecchiato¹, Marco Coppi², Claudia Niccolai²

¹Department of Experimental and Clinical Medicine (DMSC), University of Florence, Largo Brambilla, 3, Firenze (FI) 50134, Italy.

Journal of virological methods

|May 25, 2024

概括

基于废水的流行病学有效地追踪SARS-CoV-2 RNA. 在比较四种方法时,商业逆转录实时PCR (RT-PCR) 和滴滴数字PCR (ddPCR) 显示在废水中检测病毒的灵敏度更高.

科学领域:

环境微生物学环境微生物学
分子诊断学分子诊断学
公共卫生监督是对公共卫生的监督.

背景情况:

基于废水的流行病学是监测传染病传播的宝贵工具.
在废水中准确检测SARS-CoV-2RNA对于公共卫生至关重要.
了解不同检测协议的性能对于可靠的监控至关重要.

研究的目的:

为了比较三个反转录实时PCR (RT-PCR) 协议和一个滴滴数字PCR (ddPCR) 协议在废水中检测SARS-CoV-2RNA的性能.
评估各种定量检测方法的敏感性和有效性.
确定在废水中进行SARS-CoV-2RNA监测的最佳方法.

主要方法:

用四种不同的分子检测协议分析了35个废水样本.
三个协议采用了反转录实时PCR (RT-PCR).
一个协议使用滴滴数字PCR (ddPCR).

主要成果:

通过至少一种方法在85.7%的样本中检测到SARS-CoV-2RNA.
与内部RT-PCR试验相比,商业RT-PCR试验和ddPCR试验显示出更高的灵敏度.
检测率差异很大,两种方法的阳性率为51.4%,三种方法的阳性率为22.8%,所有四种方法的阳性率为8.6%.

结论:

针对不同基因的多个检测系统可以提高废水监测的灵敏度.
商业RT-PCR和ddPCR是在废水中检测敏感的SARS-CoV-2RNA的有希望的方法.
优化协议对于有效的基于废水的SARS-CoV-2流行病学至关重要.

关键词:

数字PCR是数字的PCR.滴滴数码PCR可以进行.环境监督环境监督环境监督反转录实时PCR反转录这就是SARS-CoV-2病毒.废水是废水,废水就是废水.基于废水的流行病学.

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