相关实验视频

Updated: Jun 25, 2025

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

外体LRG1通过结合FN1蛋白促进非小细胞肺癌的扩散和转移

Hao Wu¹, Chao Zeng², Guodong Wu¹

¹Department of Respiratory and Critical Care Medicine, Shenzhen Second People's Hospital, Shenzhen, 518035, China.

Experimental cell research

|May 25, 2024

概括

在非小细胞肺癌 (NSCLC) 外体中富含白的α2-糖蛋白-1 (LRG1) 促进瘤细胞转移. 向LRG1或纤维素1 (FN1) 可能提供新的NSCLC治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

富含白的α2-糖蛋白-1 (LRG1) 在多种癌症中被上调,包括非小细胞肺癌 (NSCLC).
在NSCLC细胞转移中LRG1的特定作用仍然在很大程度上未被描述.
外体,小的细胞外囊泡,涉及到癌症的进展和细胞间通信.

研究的目的:

在非小细胞肺癌 (NSCLC) 细胞转移中研究外体白丰富的α2-糖蛋白-1 (LRG1) 的作用.
阐明导致NSCLC进展的外体LRG1介导促进的分子机制.
根据LRG1及其相互作用伙伴,确定基于NSCLC治疗的潜在治疗点.

主要方法:

使用各种生化和成像技术分析NSCLC细胞衍生的外体.
试验室试验评估外体LRG1对NSCLC细胞增殖,迁移,入侵和上皮-介质细胞过渡 (EMT) 的影响.
使用动物模型进行体内研究,以评估由外体LRG1.1影响的NSCLC细胞的转移潜力.
在NSCLC细胞中识别和功能分析涉及LRG1的蛋白质-蛋白质相互作用.

主要成果:

在NSCLC细胞及其分泌的外体内,LRG1的表达很高.
外体LRG1显著增强NSCLC细胞的增殖,迁移,入侵和EMT.
含有LRG1的外体的使用在体内促进NSCLC转移.
确定了LRG1和纤维素1 (FN1) 之间的细胞质相互作用.
发现纤维素1 (FN1) 可以抵消LRG1对外体介导NSCLC细胞调节的冲击作用.

结论:

外体LRG1在促进非小细胞肺癌 (NSCLC) 细胞转移方面发挥着关键作用.
LRG1和纤维素1 (FN1) 之间的相互作用影响NSCLC细胞行为.
向外体LRG1或纤维素1 (FN1) 是NSCLC治疗的一个有希望的治疗策略.

关键词:

和入侵,入侵.异构体是什么? 异构体是什么? 外构体是什么?FN1 FN1 FN1 的意思是什么?这就是LRG1的重点.移民移民移民在NSCLCLC中,我们可以看到.核武器的扩散

更多相关视频

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Published on: June 16, 2018

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...

Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...