相关实验视频

Updated: Jun 25, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

在肠道炎症中,Atox1通过ROS-NLRP3炎症酶通路调节巨细胞两极分化

MingXian Chen^1,2, Yu Chen³, Rui Fu¹

¹Department of Gastroenterology, Tongde Hospital of Zhejiang Province, No. 234, Gucui road, Hangzhou, 310012, China.

Journal of translational medicine

|May 25, 2024

概括

抗氧化剂-1 (Atox1) 通过调节ROS-NLRP3通路,促进炎症性肠病 (IBD) 中的M1巨分化和炎症. 阿托克斯1是IBD的潜在治疗点.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
分子生物学分子生物学

背景情况:

炎症和氧化应激是炎症性肠病 (IBD) 病理生理学的关键.
铜伴侣抗氧化剂-1 (Atox1) 在肠道炎症中的作用尚不清楚.

研究的目的:

为了研究Atox1对巨细胞在肠道炎症的小鼠模型的影响.
探索Atox1在调节巨细胞极化和炎症反应中的作用.

主要方法:

使用了TNBS诱导的大肠炎小鼠模型和Atox1条件淘汰赛小鼠.
流式细胞计,ELISA,定量RT-PCR和西式涂抹评估了巨细胞特征,细胞因子水平和基因/蛋白质表达.
分析的关键标志物包括M1/M2极化标志物,p47phox,NLRP3和Caspase-1 p20.

主要成果:

在TNBS诱导的大肠炎中,巨细胞的Atox1表达和M1两极化升高.
抑制Atox1降低了ROS的产生,p47phox表达和M1极化.
在Atox1淘汰赛中,小鼠的炎症反应减弱,细胞因子产生和M1极化.
阿托克斯1的作用通过ROS-NLRP3炎症酶通路进行介导.

结论:

在肠道炎症中,Atox1促进M1巨细胞的两极分化和促炎性细胞因子的产生.
亚托克斯1通过ROS-NLRP3炎症酶通路调节巨细胞的两极分化.
亚托克斯1代表了管理炎症性肠病的潜在治疗标.

关键词:

铜的代谢铜的代谢炎症性肠病是一种炎症性肠病.巨细胞的两极分化在NLRP3炎症酶体中,NLRP3炎症这就是ROSOS ROS.

更多相关视频

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Inflammation

Inflammation