该ilv2基因,编码乙酸盐合成酶为分支链氨基酸生物合成,是需要植物病原性由Leptosphaeria maculansans
Nicholas F Chong1, Angela P Van de Wouw1, Alexander Idnurm2
1School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Molecular biology reports
|May 25, 2024
概括
抑制乙烯酸乳酸合成酶 (ALS) 除草剂可能会影响菌Leptosphaeria maculans,这是兰花的病原体. 导致真菌发生突变.
科学领域:
- 植物病理学 植物病理学
- 菌类遗传学 菌类遗传学
- 农业科学 农业科学
背景情况:
- 科拉黑腿疾病控制通常涉及杀菌剂.
- 向乙酸盐合成酶 (ALS) 的除草剂在农业中广泛使用.
- 该ALS酶对于植物和真菌中的氨基酸合成至关重要.
研究的目的:
- 为了研究ALS抑制除草剂对Leptosphaeria maculans的影响.
- 探索突变L. maculans ilv2基因的影响,该基因为ALS编码.
主要方法:
- 在体外生长测定与除草剂暴露.
- 植物疾病实验评估病变的发展.
- 在CRISPR-Cas9基因编辑中突变L. maculans.中的ilv2基因.
主要成果:
- 暴露于除草剂在体外对真菌生长的影响有限.
- 除草剂治疗减少了大麻植物的病变大小.
- ilv2基因的突变使得L. maculans没有致病性.
结论:
- 抑制ALS的除草剂可能对卡诺拉黑腿病的严重程度有很小的影响.
- 准真菌ALS酶为疾病控制提供了一个潜在的战略.
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