结合组织清除和光片成像分析癌症发病的协议在双标记器的马赛克分析中使用双标记器对小鼠模型进行癌症发病分析
Jianhao Zeng1, Elaina Ball1, Xian Zhou1
1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, VA 22908, USA.
STAR protocols
|May 26, 2024
概括
这项研究引入了一种使用组织清除和光片成像的新协议,用于分析整体鼠标组织中的前恶性克隆. 这种方法通过提高吞吐量和保存3D架构来增强癌症发病的研究.
科学领域:
- 在瘤学瘤学.
- 发展生物学 发展生物学
- 生物技术是生物技术.
背景情况:
- 用双标记 (MADM) 进行马赛克分析的小鼠模型对于研究人类癌症的克隆起源至关重要.
- 传统的克隆分析方法很费力,可能会破坏组织架构,限制研究效率.
- 需要先进的技术来以高通量和结构保存的方式分析前恶性克隆.
研究的目的:
- 提出一种优化的协议,用于分析在整体挂载的MADM标记组织中的前恶性克隆.
- 整合组织清除和光片成像,以改善克隆分析.
- 克服传统劳动密集型克隆分析管道的局限性.
主要方法:
- 使用MADM.生成使用马赛克标记的癌症小鼠模型.
- 组织采集,固定和先进的组织清除技术.
- 高分辨率光片成像和随后的整体安装组织的克隆大小分析.
主要成果:
- 该协议使得在完整的整体组织中对前恶性克隆进行分析.
- 组织清除和光片成像的整合保留了分析组织的3D架构.
- 与传统管道相比,所介绍的方法提供了一种更高效和更高吞吐量的方法.
结论:
- 该协议为研究癌症发病的克隆动态提供了一个强大的工具.
- 该技术允许对早期癌症发展进行详细的空间和定量分析.
- 该方法对推进癌症研究和药物发现具有重大意义.
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