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FGF18损害了胚胎细胞活力,DNA双链断裂和孕妇对怀孕基因的识别

André Lucio Fontana Goetten¹, Marcos Henrique Barreta¹, Yago Pinto da Silva¹

¹Laboratory of Animal Reproduction Physiology, LAFRA, Federal University of Santa Catarina, Curitibanos, SC, Brazil.

|May 26, 2024

概括

纤维细胞生长因子18 (FGF18) 通过增加DNA损伤和减少关键基因表达,显著阻碍早期牛胚胎发育. 这表明FGF18有助于牛的高胚胎死亡率.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

牛的高胚胎死亡率 (体内10-40%,体外60-70%) 与胚胎活力基因表达的减少,DNA修复的抑制和DNA损伤的增加有关.
纤维细胞生长因子18 (FGF18),在卵泡颗粒细胞中发现,已知会增加细胞亡和DNA损伤,促使研究其对卵细胞和早期胚胎死亡的影响.

研究的目的:

调查FGF18对累积膨胀,卵细胞成熟和牛胚胎早期发育 (分裂至胚芽细胞阶段) 的影响.

主要方法:

一个传统的牛体胚胎生产系统使用来自屠宰场的卵巢.
在体外成熟和/或胚胎培养过程中添加了FGF18.
评估了累积膨胀,核成熟,分裂率,胚胎细胞发育,DNA损伤 (酸化组合素H2AFX焦点) 和mRNA表达 (PTGS2,IFNT2).

主要成果:

在体外成熟过程中添加FGF18并没有影响FSH诱导的累积膨胀或核成熟率.
虽然裂变率没有受到影响,但FGF18显著抑制了胚胎细胞并扩大了胚胎细胞的发育,这表明胚胎发育延迟.
用FGF18培养的胚胎表现出更高水平的DNA损伤 (酸化组合素H2AFX焦点) 和在胚芽细胞中减少PTGS2和IFNT2mRNA的积累.

结论:

FGF18通过损害DNA双链断裂修复,在牛早期胚胎死亡中发挥作用.
FGF18对胚胎活力和孕妇怀孕的认知至关重要的基因的表达产生负面影响.
这些发现突出了FGF18作为早期牛胚胎发育的调节因素,有助于观察到的死亡率.

关键词:

修复DNA的修复DNA的修复胚胎发育的发展联邦政府财政基金.如果IFNT2是IFNT2的话.卵细胞成熟的过程

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