来自Borago officinalis L.的花蜜的超结构和发展以及其分泌中的植物化学变化
Angelo Gismondi1, Gabriele Di Marco1, Lorena Canuti1
1Dept. of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, Rome 00133, Italy.
概括
花产生的花蜜在合成时达到顶峰,花蜜的化学成分随着时间的推移而变化. 晚期花蜜中增加的黄酸可能会吸引特定的授粉者或阻止花蜜掠夺者.
科学领域:
- 植物生物学 植物生物学
- 生态生态学 生态生态学
- 生物化学 生化学
背景情况:
- 博拉吉纳科植物是已知的花蜜来源,但它们的花蜜和花蜜鲜为人所知.
- 树 (Borago officinalis L.) 是这个家族中一个常见的物种.
研究的目的:
- 调查花蜜生产动态和树中的化学成分.
- 分析波拉花蜜的超结构和形态.
- 描述树花蜜和花结构中的植物化学物质.
主要方法:
- 随着时间的推移测量花蜜分泌量.
- 传输电子显微镜 (TEM) 和扫描电子显微镜 (SEM) 用于的超结构.
- 提尼尔染色和ESI/EELS用于多糖和检测.
- 谱光测量和HPLC-DAD用于植物化学分析.
主要成果:
- 蜜汁分泌在结合时达到峰值 (5.1μL/花),并在9天内线性下降.
- 蜜蜂的运输很可能是通过阿波塑性途径发生的,通过颗粒细胞分泌物 (外细胞分裂) 释放.
- 结核体在分泌后经历退化,可能是通过自或衰老.
- 在晚期的花蜜中观察到大量的黄化合物积累,这表明它们具有功能性作用.
结论:
- 花蜜的生产和化学是动态的过程.
- 甜蜜液退化涉及编程的细胞事件.
- 晚期花蜜的黄积累可能有生态功能,影响着授粉者相互作用和植物防御.
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