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放射性骨中骨质整合不良的分子机制:在老鼠骨模型中的体内研究

Omar Omar¹, Louise Rydén², Abdel Rahman Wamied³

¹Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Journal of clinical periodontology

|May 27, 2024

概括

以前的辐射暴露会通过改变分子介质,损害植入物周围的骨愈合. 乱的炎症是阻碍骨整合和骨植入物接触的关键因素.

科学领域:

生物材料科学生物材料科学
整形外科的研究研究.
分子生物学分子生物学

背景情况:

放射治疗可能会损害骨愈合和骨质整合.
辐射后组织-植入物接口的分子机制尚未完全理解.

研究的目的:

为了确定关键的分子媒介参与辐射诱导的损害骨质整合.
为了研究先前照射对植入物周围分子环境的影响.

主要方法:

植入物被插入老鼠的小腿骨中,一些老鼠被预先暴露在20 Gy辐射中.
分析包括组织形态测量,生物力学,qPCR和植入后1周和4周的ELISA.

主要成果:

辐射减少了骨植入物接触和移除扭矩.
辐射在植入细胞中增加了促炎性 (Tnf) 和骨性 (Ctsk) 基因表达,同时降低了骨形成 (Alpl) 基因表达.
周围植入的骨显示骨形成基因表达增加 (Alpl,Bglap),软组织表现出增加的亲炎性 (Tnf) 和亲纤维性 (Tgfb1) 基因表达.
在被辐射的老鼠中,血清中炎症,骨形成和骨再吸收蛋白质的水平升高.

结论:

辐射破坏了骨质整合所必需的多种生物过程.
扰乱的炎症似乎是辐射对植入部位骨愈合的负面影响的核心机制.

关键词:

纤维化纤维化基因表达的基因表达方式这是一种炎症炎症炎症炎症.口腔植入物是一种口腔植入物.辐射疗法辐射疗法

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