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风中的低语:面罩采样用于在患有肺结核的儿童中检测Mycobacterium结核病
Lennard Meiwes1,2,3, Irina Kontsevaya1,2,3,4, Dumitru Chesov1,5
1Clinical Infectious Diseases, Research Centre Borstel, Borstel, Germany.
面罩采样 (FMS) 在体外显示了Mycobacterium结核病DNA的低检测极限. 然而,FMS没有检测到儿童的结核病DNA,这表明它可能不会改善儿科诊断.
科学领域:
- 微生物学 微生物学
- 儿童传染病 儿童传染病
- 诊断发展 诊断发展
背景情况:
- 面罩采样 (FMS) 在患有结核病的成年人中检测到Mycobacterium结核病DNA.
- 没有研究评估了FMS用于检测儿童肺结核.
- 在儿童中开发了一种针对M.结核病特异性DNA的FMS方法.
研究的目的:
- 评估FMS在儿童肺结核检测中的可行性.
- 为了评估新开发的FMS方法在儿科患者群体中的有效性.
主要方法:
- 面罩上添加了M.结核病,并使用GeneXpert-Ultra,定量PCR和下一代定向测序进行分析.
- 患有肺结核的10名儿童在30分钟内戴上了三个修改后的FFP2口罩.
- 这些口罩被分析为M.结核病特异性DNA.
主要成果:
- 在体外实验表明,在GeneXpert-Ultra.上对M.结核病的检测极限为3.75个殖民地形成单位.
- 在患有肺结核的儿童所佩戴的30个口罩中,没有检测到M.结核病特异性DNA.
- 儿科FMS方法在体外表现出低检测极限.
结论:
- 儿童面具采样在体外对M.结核病DNA的检测极限较低.
- 该研究表明,FMS在目前的形式中,可能不比现有的方法更有效地检测儿童的M.结核病.
- 可能需要进一步的研究来优化FMS用于儿科结核病诊断.
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