相关实验视频

Updated: Jun 25, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

双硫化制约Fabs克服了高分辨率单颗粒冷EM的目标尺寸限制

Jennifer E Kung¹, Matthew C Johnson¹, Christine C Jao¹

¹Department of Structural Biology, Genentech Inc., South San Francisco, CA 94080, USA.

bioRxiv : the preprint server for biology

|May 27, 2024

概括

研究人员开发了Rigid-Fabs,即形状刚性支架,以实现小蛋白质的高分辨率冷电子显微镜 (cryo-EM). 这一突破克服了在确定疾病相关小蛋白质结构方面的局限性.

科学领域:

结构生物学结构生物学
生物化学生化学
生物物理学的生物物理.

背景情况:

高分辨率的蛋白质结构对于理解生物机制和药物发现至关重要.
虽然冷电子显微镜 (cryo-EM) 对大蛋白质非常出色,但由于信号对噪声和对齐问题较低,小蛋白质 (<50 kDa) 仍然具有挑战性.
使用灵活支架的现有方法往往导致目标蛋白质的分辨率较差.

结论:

开发了一种创新的方法,用于为冷EM创建刚性抗体碎片支架 (Rigid-Fabs).
成功实现了以前难以处理的小蛋白质的高分辨率冷EM结构确定.
建立了一种通用方法来扩大冷EM的范围,用于研究与人类疾病相关的小蛋白质.

更多相关视频

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...