外源性脂质囊泡诱导细胞内激酶介导的细胞聚合在动物的近亲单细胞的亲戚
bioRxiv : the preprint server for biology
|May 27, 2024
概括
卡普萨斯波拉 (Capsaspora owczarzaki) 原生动物的聚合是由多种脂质触发的,需要克拉特林介导的内细胞分裂. 这一发现提供了对动物进化和杆菌病控制的见解.
科学领域:
- 进化生物学 进化生物学
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
背景情况:
- 一种原生动物Capsaspora owczarzaki表现出化学调节的聚合行为.
- 这种行为类似于动物细胞聚合,可能对多细胞进化至关重要.
- 卡普萨斯波拉的聚合可能会通过影响寄生虫宿主殖民来影响istosomiasis的传播.
研究的目的:
- 为了阐明调节Capsaspora聚合的分子机制.
- 为了确定激活聚合的脂质线索的化学特性.
- 了解参与Capsaspora聚合的细胞过程.
主要方法:
- 确定激活Capsaspora聚合的脂质线索的化学特性.
- 研究了克拉特林介导的内细胞分裂在Capsaspora聚合中的作用.
- 评估了细胞-细胞粘附的调节机制 (转录与转译后).
主要成果:
- 发现各种丰富的zwitterionic脂质可以诱导Capsaspora聚合.
- 在Capsaspora中的聚合被证明需要克拉林介导的内细胞分裂.
- 卡普萨斯波拉聚合是由细胞-细胞粘附的翻译后激活启动的,而不是转录调节.
结论:
- 卡普萨斯波拉聚合可以通过各种脂质丰富的条件来激活.
- 对于克拉特林介导的内细胞结合的要求强调了内细胞结合相关信号在早期动物进化中的重要性.
- 细胞-细胞粘附的翻译后调节为Capsaspora聚合提供了关键的分子洞察力,对进化和医学研究都有影响.
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.5K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.5K
Receptor-mediated Endocytosis
104.4K
Overview
104.4K
Clathrin Coated Vesicles
6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K
Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K
Fusion of Secretory Vesicles with the Plasma Membrane
11.0K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.0K
Introduction to Membrane Traffic
7.1K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.1K


