在感染期间,ILC2s通过特定阶段的S1P受体导航组织再分配
bioRxiv : the preprint server for biology
|May 27, 2024
概括
组织居住的淋巴细胞,如肠道第2组先天性淋巴细胞 (ILC2s),通过淋巴血管重新循环. 它们的迁移依赖于特定的氨酸-1-酸盐受体 (S1PRs) 来输出组织并进入血液.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 淋巴细胞贩运 淋巴细胞贩运
背景情况:
- 循环淋巴细胞向感染部位的招募是众所周知的.
- 组织居民淋巴细胞循环的机制仍然不太清楚.
- 第二组先天性淋巴细胞 (ILC2s) 存在于组织中,可以重新分布.
研究的目的:
- 为了阐明肠道ILC2循环的分子基础.
- 在虫感染或IL-25刺激期间识别参与ILC2迁移的关键分子.
主要方法:
- 研究了对虫感染或IL-25的反应中的ILC2再分配.
- 分析了淋巴血管网络在ILC2迁移中的作用.
- 在ILC2s.中检查了表观遗传变化和sphingosine-1-phosphate受体 (S1PR) 表达.
- 评估了S1PR1和S1PR5在ILC2从组织和淋巴结出口中的功能.
- 研究了CD69对S1PR1功能的影响.
主要成果:
- ILC2的再分配需要进入淋巴血管网络.
- 刺激IL-25诱导表观遗传变化和S1PR表达在肠道ILC2s.
- S1PR5对于ILC2从肠道组织进入淋巴的流出至关重要.
- S1PR1调解了ILC2从淋巴结进入血液循环的过程.
- 通过引起S1PR1内部化,CD69表达影响S1PR1依赖迁移.
结论:
- 不同S1P受体的特定阶段要求控制着ILC2的再分配.
- ILC2迁移涉及不同的S1PRs离开组织而不是进入循环.
- 天生的和适应性淋巴细胞共享血管网络,但使用专门的迁移线索.
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