在早期胚胎中,FoxO/DAF-16的模式,调节和作用
bioRxiv : the preprint server for biology
|May 27, 2024
概括
叉头盒O (FoxO) 转录因子对于早期胚胎发育至关重要. 在C. elegans中,FoxO/DAF-16对生殖细胞发育和细胞周期的节奏至关重要,独立于胰岛素信号传递.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 胰岛素抵抗和糖尿病与妊娠并发症和生育问题有关,但胰岛素信号在早期胚胎发育中的作用尚不清楚.
- 胰岛素/胰岛素类生长因子信号传递 (IIS) 途径的目标,分叉家族转录因子FoxO (C. elegans中的DAF-16),是一个关键参与者.
- 了解FoxO在胚胎发生中的作用对于破译代谢障碍的发育影响至关重要.
研究的目的:
- 为了研究胰岛素/类似胰岛素的生长因子信号传导 (IIS) 途径目标,FoxO/DAF-16,在早期的C. 伊莱根斯 (elegans) 的意思是胚胎发生.
- 确定早期胚胎中FoxO/DAF-16的空间和时间模式.
- 阐明FoxO/DAF-16在生殖系发展中的调节机制和功能意义.
主要方法:
- 在早期C.中对内源标记的FoxO/DAF-16进行定量实时成像. 优雅的胚胎.胚胎.
- 对FoxO/DAF-16核定位动态的分析.
- 功能性试验评估FoxO/DAF-16对细胞周期进展的影响.
主要成果:
- FoxO/DAF-16在2-4个细胞阶段的胚胎中显示出均的核定位,从8个细胞阶段的胚胎中丰富了胚胎前体细胞核.
- 核丰富需要细菌命运规范,PI3K/PTEN信号和USP7,但独立于胰岛素受体和AKT.
- FoxO/DAF-16 作为细胞循环节奏剂,其缺失导致细胞循环缩短,特别是在胚胎细胞中.
结论:
- 在早期的C. elegans胚胎发生过程中,FoxO/DAF-16表现出特定的生殖系模式.
- 它的核定位受细菌命运,脂信号和USP7的调节,独立于正规的胰岛素受体信号.
- 福克斯O/DAF-16在节奏胚胎细胞周期中发挥着关键作用,突出了其在发育时间表中的重要性.
相关概念视频
Determination
18.5K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.5K
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Cleavage and Blastulation
45.1K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
45.1K


