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下一代测序与常规培养方法之间的比较分析,以检测与empyema相关的微生物:系统性审查

Indra Yovi^1,2,3,4, Nur A Syah⁵, Dewi Anggraini^2,3,6

¹Doctoral Program of Biomedicine, Faculty of Medicine, Universitas Andalas, Padang, Indonesia.

|May 27, 2024

概括

下一代测序 (NGS) 比传统的培养方法更有效地识别引起 empyema 的细菌,即使在培养负的病例中. 这种先进的技术为这种全球健康问题提供了更准确的诊断.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
诊断技术诊断技术的使用

背景情况:

肺炎是全球重要的健康问题,在细菌识别方面存在挑战.
传统的肺部液体培养方法在准确检测致病原体方面可能存在局限性.

研究的目的:

为了比较下一代测序 (NGS) 的诊断准确性与常规的肺部液体培养,以识别引起 empyema 的细菌.

主要方法:

在五个数据库 (谷歌学者,科学直接,柯克兰,研究门,PubMed) 进行了系统的文献搜索.
包括将传统培养与NGS进行对比的研究,以识别Empyema病原体.
提取了有关阳性细菌识别和概况的数据;使用Joanna-Briggs Institute (JBI) 工具评估了研究质量.

主要成果:

分析中包括了3项涉及354名患者的研究.
与传统培养相比,下一代测序在检测引起的细菌方面表现优越.
NGS确定了常见的病原体,包括*Streptococcus pneumoniae*,*Staphylococcus aureus*和无氧细菌,特别是在培养负样本中.

结论:

下一代测序是一种比传统培养更有效的诊断工具,用于识别empyema病原体.
建议使用更大的样本大小和更广泛的病原体分析进行进一步的研究,以支持NGS在empyema诊断中的广泛采用.

关键词:

恩皮埃马 (Empyema) 是一种病.文化文化文化文化诊断诊断诊断的诊断诊断诊断诊断的诊断这是一个metagenomic.这是下一代测序.

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