相关实验视频
Updated: Jun 25, 2025

06:16
mirMachine: A One-Stop Shop for Plant miRNA Annotation
Published on: May 1, 2021
2.5K
在果和Valsa mali之间的组织特异相互作用中,miRNAs/milRNAs的自适应调节
Chengyu Gao1, Binsen Zhao1, Jian Zhang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Horticulture research
|May 27, 2024
概括
这项研究揭示了微RNA (miRNA) 和微RNA类RNA (milRNA) 如何调节组织特异的植物病原体相互作用. 它识别了特定的miRNAs和milRNAs,这些miRNAs和milRNAs控制了果耐药性和树皮和叶子组织中的真菌病原性.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 植物病原体相互作用表现出组织特异性,但微RNA/微RNA类RNA (miRNA/milRNA) 在这种调节中的作用尚不清楚.
- 了解这些分子机制对于开发抗病作物至关重要.
研究的目的:
- 研究果miRNAs和Valsa mali (Vm) milRNAs在组织特异相互作用中的差异表达和功能.
- 为了确定特定的miRNAs/milRNAs及其参与调节果耐药性和Vm病原性的目标基因.
主要方法:
- 在被Vm感染的果树皮和叶子中对miRNAs/milRNAs及其标基因的差异表达分析.
- 通过短暂表达和沉默实验,对已识别的miRNAs/milRNAs (Mdo-miR482a,Vm-milR57) 和目标基因 (NLR,病原性基因) 的功能验证.
主要成果:
- 在Vm感染期间,果miRNAs和Vm milRNAs都在树皮和叶子中显示出不同的表达模式.
- Mdo-miR482a针对果的NLR基因,其调节影响了果的耐药性;Vm-milR57针对Vm的病原性基因,其下调增强了病原性.
结论:
- 这项研究首次证明了植物和病原体miRNAs/milRNAs在Valsa mali-apple相互作用中的组织特异性作用.
- 这些发现为控制特定组织的植物和真菌相互作用的适应性调节机制以及疾病管理的潜在目标提供了新的见解.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Regulation of Expression Occurs at Multiple Steps
22.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Regulation of Expression at Multiple Steps
892
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
892
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K

