在印度西部和中部传播的传染病病毒的分离和分子特征
A A Agnihotri1, S P Awandkar2, R C Kulkarni3
1Post-graduate Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary & Animal Sciences, Maharashtra Animal & Fishery Sciences University, Udgir 423517, India.
Iranian journal of veterinary research
|May 27, 2024
概括
印度的传染病 (IBD) 爆发是由各种非常有毒的IBDV菌株引起的,导致家禽的大量损失. 这项研究对这些场隔离物进行了表征,以了解疾病.
科学领域:
- 兽医病毒学 兽医病毒学
- 鸟类病理学 鸟类病理学
- 分子流行病学分子流行病学
背景情况:
- 传染病 (IBD) 在2020-2021年期间在印度中部和西部造成了重大经济损失.
- 由于IBDV的爆发,家禽养殖面临着巨大的挑战.
研究的目的:
- 鉴定印度现场疫情中的传染性病病毒 (IBDV) 隔离物.
- 为了确定负责最近疾病流行病的特定IBDV菌株.
主要方法:
- 分析了来自42个家禽群的360个样本.
- 组织病理学,分子检测和部分VP2基因的遗传学类型.
- 反转录聚合酶链反应 (RT-PCR) 用于病毒检测和测序.
主要成果:
- 观察到高死亡率 (15.25-60.18%) 和特征性的胸口病变.
- 在培养分离物中观察到的细胞病变效应,包括细胞胀,圆化和碎片化.
- 遗传学分析在现场分离物中发现了19种非常毒的IBDV (vvIBDV) 菌株和3种经典菌株.
结论:
- 多种vvIBDV菌株被确定为印度中部和西部IBD爆发的致病原体.
- 了解循环IBDV菌株对于有效的疾病控制策略至关重要.
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