Circ_0007432通过SRSF1/KLF12轴促进非小细胞肺癌的进展和巨细胞M2极化
Shanshan Mao1,2, Dongyu Wu1, Xiaozhen Cheng2
1Radiotherapy Department, The First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, P.R. China.
iScience
|May 27, 2024
概括
循环RNAcirc_0007432通过增强细胞活力,迁移和M2巨细胞极化,促进非小细胞肺癌 (NSCLC) 的进展. 它稳定KLF12,导致IL-8释放增加,导致恶性行为.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 非小细胞肺癌 (NSCLC) 是全球癌症相关死亡的主要原因.
- 了解NSCLC中circRNAs的特定功能对于开发向疗法至关重要.
研究的目的:
- 研究circ_0007432在非小细胞肺癌 (NSCLC) 进展中的作用和机制.
- 为了阐明涉及circ_0007432,SRSF1,KLF12和IL-8在NSCLC发病过程中的分子相互作用.
- 评估circ_0007432对瘤生长和M2巨细胞极化的影响.
主要方法:
- RNA结合蛋白免疫沉降 (RIP) 和染色体免疫沉降 (ChIP) 试验.
- 电泳运动移动转移试验 (EMSA) 和双光酶记者试验.
- 在体内瘤生长研究和救援实验,以验证分子相互作用和功能作用.
主要成果:
- 在体内,Circ_0007432显著促进NSCLC细胞活力,迁移,入侵和瘤生长.
- Circ_0007432增强了M2巨细胞的两极分化,并抑制了NSCLC细胞的亡.
- 在机械上,circ_0007432通过招募SRSF1来调节KLF12,这反过来又促进IL-8的表达和释放.
结论:
- 在NSCLC中,Circ_0007432通过调节SRSF1/KLF12/IL-8信号轴作为瘤促进剂.
- 这种circRNA通过M2巨分化影响癌细胞恶性和瘤免疫微环境.
- 针对circ_0007432/SRSF1/KLF12/IL-8通路可能为NSCLC提供一种新的治疗策略.
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