利用真菌多样性的价值来降解化诺
Imen Akrout1,2, Karima Staita1,2, Hèla Zouari-Mechichi1
1Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, 3038 Sfax, Tunisia.
Heliyon
|May 27, 2024
概括
溶性真菌,特别是Bjerkandera adusta和Porosterum spadiceum,可以生物降解诺基诺抗生素,减少环境污染和细菌耐药性. 这项研究强调了真菌在抗生素清除中的潜力.
科学领域:
- 环境微生物学环境微生物学
- 生物修复是一种生物修复.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 广泛使用抗生素,特别是诺基诺,通过促进细菌耐药性和破坏生态系统,造成环境风险.
- 了解生物降解途径对于减轻抗生素污染至关重要.
研究的目的:
- 为了研究十种化诺类抗生素的生物降解效率,通过各种化菌.
- 为了识别具有抗生素去除高潜力的真菌菌株.
- 探索参与诺基诺降解的酶机制.
主要方法:
- 选九种林基诺化真菌物种,以检测它们降解十种不同的诺基诺抗生素的能力.
- 在15天的时间内量化降低抗生素度的量化.
- 分析剩余的抗生素活性和抗菌代谢物的潜在形成.
- 探索酶降解途径 (乳酸酶,过氧化酶,P450细胞染色体) 和生物质吸附.
主要成果:
- 在真菌菌株之间观察到生物降解效率的显著差异.
- Bjerkandera adusta和Porosterum spadiceum的降解率最高,分别消除了八种和六种抗生素.
- 此外,Trametes versicolor和Pleuroteus ostreatus也表现出有效的降解,其中T. versicolor降解了六种基诺的70%.
- 剩余的抗生素活性有时与度降低无关,这表明生物活性代谢物的形成.
- 通过乳酶,过氧酶和P450细胞染色体的酶降解以及生物质吸附被确定为潜在的机制.
结论:
- 红素溶解性真菌具有显著的生物降解诺基诺抗生素的潜力.
- 像B. adusta和P. spadiceum这样的特定菌株在降低诺基诺度方面非常有效.
- 对真菌酶途径的进一步研究可以导致针对抗生素污染的新生物修复策略.
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