牙材料类型和力对牙周炎衍生的下微生物群的影响
Carolina Montoya1, Divyashri Baraniya2, Tsute Chen3
1Smart Biomaterials Laboratory, Department of Oral Health Sciences, Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA.
Biofilm
|May 27, 2024
概括
牙科生物材料显著改变牙下微生物组. 抗微生物复合物控制了生物膜活力,而酸在静态条件下促进了失活性,而循环负荷反转效应.
科学领域:
- 牙科材料科学 牙科材料科学
- 微生物学 微生物学
- 牙周病研究 牙周病研究
背景情况:
- 修复性牙科材料可以在牙线以下容纳微生物,通过改变口腔微生物组,可能导致感染.
- 牙科材料对牙下微生物组组成的影响仍未得到充分探索.
研究的目的:
- 评估树脂牙复合材料 (COM),抗微生物压电复合材料 (BTO) 和酸 (HA) 的微生物调节特性.
- 评估静态和循环负荷 (模拟口) 对这些生物材料生物膜形成和微生物组合的影响.
主要方法:
- 使用了来自牙周病患者的体外膜下微生物组模型.
- 在生物膜生长过程中,在静态和循环负荷条件下测试了三种生物材料 (COM,BTO,HA).
- 使用16S rRNA基因测序分析了微生物组的组成,并通过subgingival微生物失调指数 (SMDI) 量化了失调.
主要成果:
- 循环加载通常会增加生物膜的活力,除了抗菌复合材料 (BTO).
- 静态条件导致氧酸盐 (HA) 上的生物膜生物质较高,并增加了失活性,丰富了像 Porphyromonas gingivalis.这样的周围病原体.
- 在HA和复合材料之间,微生物组的多样性有显著差异. 循环加载逆转了HA失调,但在复合材料上增加了失调,丰富了P. gingivalis和Fusobacterium nucleatum.
结论:
- 牙科生物材料显著调节下微生物群,影响口腔感染的动态.
- 抗微生物压电复合材料证明了在不破坏主要物种的情况下控制微生物群生存的潜力.
- 在静态负荷下,氧酸盐促进了失生,突出显示了对口腔生物膜形成和致病性的特定物质效应.
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