牙斑块微生物组的表征和功能预测在带有膜裂隙的患者中
Yuehua Zhang1,2, Qiang Zhi3,4, Jiajun Shi1,2
1Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Frontiers in cellular and infection microbiology
|May 27, 2024
概括
气膜裂 (AC) 改变了口腔细菌,在患者中丰富了特定的属性. 虽然骨移植没有改变微生物的功能,但它减少了AC患者和对照组之间的差异.
科学领域:
- 微生物学 微生物学
- 口腔健康 口腔健康
- 遗传学 是一个遗传学.
背景情况:
- 膜裂 (AC) 是一种常见的先天性缺陷,与唇裂 (CLP) 相关.
- 患有AC的患者表现出增加的口腔疾病率,但具体的微生物变化仍然不清楚.
- 膜骨移植 (ABG) 是一种常见的治疗方法,通常在青春期进行.
研究的目的:
- 系统地表征膜上方斑块微生物群在带有膜裂隙的个体中.
- 为了比较AC患者,ABG后患者和两个年龄组的健康对照之间的微生物组成.
- 了解与膜裂相关的斑块生态和微生物学.
主要方法:
- 在青少年和年轻成年人年龄组招募了87名受试者.
- 从各种队伍收集牙斑样本:AC青少年 (AAP),后ABG青少年 (PAP),健康对照 (CAP),AC年轻人 (AYP),后ABG年轻人 (PYP) 和健康对照 (CYP).
- 进行了16S rRNA基因测序和Bugbse表型预测.
主要成果:
- AC患者的微生物组成与健康对照有显著差异.
- 在LEfSe分析中,在AAP (例如,Neisseria,Haemophilus) 和AYP (例如,Capnocytophaga,Rhodococcus) 中发现了特定的细菌丰富.
- 表型分析显示了AYP中氧化应激耐受性的差异;ABG减少了差异性属,但没有改变功能表型.
结论:
- 这项研究提供了膜裂患者中supragingival斑块微生物群的系统性表征.
- 这些发现增强了对膜裂背景下的斑块生态学和微生物学的理解.
- 膜骨移植会影响微生物特征,减少特定的细菌差异.
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