相关实验视频

Updated: May 6, 2026

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

通过生物信息分析和细胞分析对Smad4核出口SUMOylation的影响

Feng Zhang¹, Jun Yang², Yifei Cheng²

¹Department of Pharmacy, Huashan Hospital, Fudan University, Shanghai, China.

Biomolecules & biomedicine

|May 27, 2024

概括

小型乌比基类修饰剂 (SUMOylation) 调节细胞活动. 这项研究确定了Ran GTPase激活蛋白1 (RANGAP1) 作为质瘤的预后标志物,对瘤生长和TGF-β/Smad信号传递至关重要.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

小型乌比基类修饰剂 (SUMOylation) 失调与质瘤有关.
识别关键的基因和通路对于理解质瘤病因和进展至关重要.

研究的目的:

为了研究SUMOylation相关基因在质瘤中的作用.
为了确定质瘤的预后标志物.
为了阐明Ran GTPase激活蛋白1 (RANGAP1) 在质瘤中的功能.

主要方法:

对TCGA Glioma和GSE16011数据集的生物信息分析.
定量实时聚合酶连锁反应 (qRT-PCR),西部涂抹 (WB) 和免疫沉 (IP) 用于RANGAP1验证.
细胞检测包括增殖,迁移,入侵,细胞亡,细胞循环分析和免疫光.

主要成果:

RANGAP1被确定为一个枢纽基因和质瘤的关键预后标志物.
在质瘤细胞中,RANGAP1的表达被降低了;它的恢复阻碍了瘤的生长.
沉默RANGAP1增强了质瘤细胞的扩散,入侵和迁移.
SUMO1与RANGAP1特别结合,影响Smad4局部化和TGF-β/Smad信号传递.
RANGAP1的SUMOylation影响细胞增殖和迁移,通过SUMOylation损害突变得到证实.

结论:

RANGAP1是质瘤的潜在预后标志物.
RANGAP1在调节质瘤细胞增殖,迁移和入侵方面发挥着重要作用.
RANGAP1的SUMOylation对于调节TGF-β/Smad信号通路至关重要,影响瘤进展.

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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

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