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Updated: Jun 25, 2025

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

血红蛋白变异与6种常用的HbA1c方法HbA1c测量的干扰

Mingyang Li¹, Song Ge¹, Xin Shu²

¹Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China.

Laboratory medicine

|May 27, 2024

概括

常见的血红蛋白变体如HbS,HbC,HbD和HbE通常不会干扰大多数HbA1c测量系统. 然而,高胎儿血红蛋白 (HbF) 显著影响所有测试方法的准确性.

科学领域:

临床化学临床化学
实验室医学实验室医学
糖尿病诊断诊断糖尿病诊断

背景情况:

糖化血红蛋白 (HbA1c) 是糖尿病的关键诊断和监测标志物.
常见的血红蛋白变体可能会干扰HbA1c测试的准确性.
评估干扰对于可靠的糖尿病管理至关重要.

研究的目的:

评估常见的血红蛋白变体对广泛使用的HbA1c测量系统的干扰.
在遇到 Hb 变异时,比较不同 HbA1c 测试方法的性能.

主要方法:

测试了六种HbA1c测量系统:2个HPLC,1个CE,1个免疫测试,1个酶测试,1个酸盐亲和力测试方法.
分析了变异和非变异样本,包括具有HbS,HbC,HbD,HbE和HbF的样本.
结果与国际临床化学联合会 (IFCC) 校准的参考方法进行了比较.

主要成果:

大多数测试方法没有显示出HbS,HbC,HbD和HbE变体的显著干扰.
在HLC-723 G8标准模式下,出现了临床上显著的干扰.
所有方法都显示出高胎儿血红蛋白 (HbF) 的负偏差,随着HbF度的增加而恶化.

结论:

胎儿血红蛋白 (HbF) 升高对各种平台的HbA1c测量准确性构成重大挑战.
酸盐亲和力,免疫检测和酶检测方法特别受到高HbF水平的影响.
需要仔细考虑潜在的干扰,特别是来自HbF的干扰,才能准确地报告HbA1c.

关键词:

在HPLC中使用HPLC.酸的亲和关系酸的亲和关系电光泳是一种电光泳.酵素酶是一种酶.这是胎儿血红蛋白.血红蛋白阿尔克阿尔克血红蛋白血红蛋白变体的血红蛋白变体免疫测试是一种免疫测试.

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