多中心对Etest,Vitek2和BD Phoenix与汁微稀释进行比较,以测试Streptococcus pneumonia的β-乳酸盐敏感性
Steven Martens1, Lize Cuypers1,2, Florian Bélik3
1National Reference Center for Invasive Pneumococci, University Hospitals Leuven, Herestraat, 49, Leuven, 3000, Belgium.
概括
维特克®2和BD尼克斯TM可靠地确定肺炎链球菌对青素,安培素和毒素的敏感性. 在某些板上,Etest®可能会低估青素和安培素的最小抑制度 (MIC),因此需要谨慎解释.
科学领域:
- 临床微生物学 临床微生物学
- 抗微生物耐药性 抗微生物耐药性
- 细菌诊断 细菌诊断是一种细菌诊断.
背景情况:
- 准确的抗微生物敏感性测试 (AST) 对于指导治疗Streptococcus pneumoniae感染至关重要.
- 几种自动化系统和梯度扩散方法用于AST,但它们的性能可能有所不同.
研究的目的:
- 评估Etest®,Vitek®2和BD PhoenixTM系统的性能,以确定S. pneumoniae对青素,安培素和胺的敏感性.
主要方法:
- 在比利时四个实验室中测试了60种具有不同MIC的独特S. pneumoniae菌株.
- Etest®,Vitek®2和BD PhoenixTM与Sensititre®微稀释 (BMD) 作为参考方法进行了比较.
- 使用EUCAST非脑膜炎断点来解释MIC结果.
主要成果:
- 维特克®2和BD PhoenixTM显示出对青素,安培素和塞福胺的高分类一致性 (CA).
- Etest®对青素和青素的CA较低,特别是在氧化板上,对于基青素,基本协议 (EA) 低至58.3%.
- 在Oxoid板上的Etest®对cefotaxime (79.2%) 的CA较低.
结论:
- 维特克®2和BD PhoenixTM可靠地进行准确的S. pneumoniae对青素,安培素和毒素的敏感性测试.
- 在Oxoid板上检测zylpenicillin和ampicillin的Etest®可能会低估MIC,因此在临床断点附近需要仔细解释.
更多相关视频
12:08Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
Published on: February 14, 2022
2.7K
09:07Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
Published on: May 24, 2024
867
相关概念视频
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...