研究牛奶包装中常见的迁移物质对蛋白酶的影响:多谱和分子对接方法
Zijian Xiong1, Yue He2, Weiyan Guan1
1College of Packaging Engineering, Jinan University, Zhuhai, Guangdong 519070, China.
牛奶包装中的常见迁移物,如酸和胺,可以抑制消化蛋白酶,素和素. 这些相互作用是自发的,外热的,由键和范德瓦尔斯力驱动,影响酶活性和结构.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 牛奶包装材料可以释放移民进入食物.
- 消化蛋白酶对于营养吸收至关重要.
- 了解迁移蛋白酶相互作用对于食品安全至关重要.
研究的目的:
- 为了研究常见的牛奶包装移民对消化蛋白酶活性的影响.
- 阐明石酸和石胺对素和素的抑制机制.
- 评估这些相互作用的结合亲和力和热力学参数.
主要方法:
- 酶活性测定 (素和素).
- 光谱技术 (光,圆形二重化).
- 分子对接模拟. 分子对接模拟.
主要成果:
- 石灰酸和石灰胺显著降低了素活性;石灰酸也降低了素活性.
- 抑制机制是多种多样的:可逆混合的酸-trypsin,不具有竞争力的酸/stearamide-pepsin.
- 结合是自发的,外热的,由键/范德瓦尔斯力驱动,具有中等的结合亲和力 (约为10^4 L/mol).
- 移民改变了蛋白质酶的光和二次结构,酸增加了在素的Tyr残留物附近的疏水性.
结论:
- 常见的牛奶包装移民可以抑制关键的消化蛋白酶.
- 结合相互作用在热力学上是有利的,并影响酶的结构和功能.
- 调查结果支持根据食品安全考虑,明智地选择牛奶包装材料.
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