相关实验视频

Updated: Jun 25, 2025

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Published on: November 14, 2014

miR-4685-3p通过调节MMP9减轻人脑微血管内皮细胞损伤

Dan Ma^1,2, Yongting Lu¹, Hong Ye²

¹Neurology Department, Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China.

Annals of clinical and laboratory science

|May 27, 2024

概括

微RNA miR-4685-3p,可以通过准矩阵金属蛋白酶-9 (MMP9) 来保护大脑微血管内皮细胞损伤. 这一发现表明大脑微型血液 (CMBs) 具有潜在的治疗作用.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

大脑微出血 (CMB) 是大脑小血管疾病 (CSVD) 的标志.
了解CMB背后的分子机制对于开发有效的治疗方法至关重要.

研究的目的:

研究miR-4685-3p在脑微血管内皮细胞损伤中的作用.
阐明miR-4685-3p调节矩阵金属蛋白酶-9 (MMP9) 表达的机制.

主要方法:

高通量测序在CMB患者中确定了差异表达的miRNA.
脂聚糖 (LPS) 诱导的人类大脑微血管内皮细胞 (hCMEC/D3) 的损伤.
定量PCR,西部斑点,CCK-8,TUNEL和双露西法酶试验评估了miRNA和MMP9的表达,细胞活力,细胞亡和血管生成.

主要成果:

在CMB患者和LPS诱导的hCMEC/D3细胞中,miR-4685-3p的下调,而MMP9的上调.
miR-4685-3p上调降低了细胞亡,增强了受伤细胞的活力和管状形成.
miR-4685-3p直接准并降低MMP9的调节,扭转LPS引起的损害.

结论:

上调 miR-4685-3p通过MMP9轴减轻LPS诱导的大脑微血管内皮细胞损伤.
miR-4685-3p可能在脑微血病 (CMBs) 的进展中起到保护作用.

关键词:

大脑微型出血.在LPS中使用LPS.在MMP9中,MMP9是MMP9.在 hCMEC/D3 中,在 miR-4685-3p 中使用.

更多相关视频

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Published on: July 14, 2020

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...