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A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
Published on: January 27, 2019
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[在使用抗生素后患有败血症的早产婴儿中应用元基因组下一代测序技术]
Chun-Yan Lou1, Yu-Ning Liu1, Xuan Zhang1
1Department of Neonatology, Henan Provincial People's Hospital/Zhengzhou University People's Hospital, Zhengzhou 450003, China.
概括
甲基因组下一代测序 (mNGS) 为早产婴儿的败血症提供了比血液培养更高的病原体检测率,即使在使用抗生素后. 这种先进的诊断工具有助于及时有效地调整治疗.
科学领域:
- 新生儿医学 新生儿医学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断
背景情况:
- 败血症是早产婴儿的关键问题,通常因先前的抗生素暴露而复杂化.
- 准确的病因诊断对于指导这种脆弱人群的适当抗菌疗法至关重要.
- 传统的诊断方法,如血培养,具有局限性,特别是在接受抗生素的婴儿中.
研究的目的:
- 评估超基因组下一代测序 (mNGS) 对早产婴儿败血症的诊断价值.
- 为了比较mNGS的病原体检测率与早产婴儿患有败血症的血液培养,特别是在抗生素治疗后.
主要方法:
- 对45名被诊断患有败血症的早产婴儿进行了回顾性分析.
- 所有婴儿在测试前至少接受了3天的抗生素治疗.
- 通过血液培养和血液mNGS检测病原体的比较.
主要成果:
- 与血液培养 (4%) 相比,血液mNGS的病原体检测率显著更高 (44%).
- mNGS识别了各种各样的病原体,包括细菌和真菌,无论抗生素治疗持续时间 (>10天或≤10天).
- 基于mNGS结果的治疗计划调整导致了85%的疗效率.
结论:
- 血液mNGS是一种更敏感的诊断工具,用于识别患有败血症的早产婴儿的病原体,即使在抗生素暴露后也是如此.
- 由于先前使用抗生素的持续时间,mNGS的有效性不会下降.
- 当临床怀疑败血症很高,但血液培养结果阴性时,应考虑mNGS进行病因诊断.
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