CircSLC25A16通过miR-335-5p/CISD2轴促进非小细胞肺癌的发展
1Department of Respiratory Medicine, Yiwu Fuyuan Private Hospital, Yiwu City, China.
Thoracic cancer
|May 28, 2024
概括
循环RNA circSLC25A16促进非小细胞肺癌 (NSCLC) 的进展,通过海绵式微RNA-335-5p (miR-335-5p) 来调节CDGSH铁硫域含蛋白2 (CISD2). CircSLC25A16可能是NSCLC治疗的新生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 非小细胞肺癌 (NSCLC) 具有显著的发病率和死亡率.
- 循环RNAs (circRNAs) 参与NSCLC的发生.
- 在NSCLC中circSLC25A16的特定作用仍未明确.
研究的目的:
- 研究circSLC25A16在NSCLC进展中的机制.
- 在NSCLC中探索circSLC25A16,miR-335-5p和CISD2之间的关系.
主要方法:
- 定量实时PCR和西斑用于评估表达水平.
- 细胞增殖,细胞亡,迁移,葡萄糖吸收和乳酸生产的测试.
- 双 luciferase 记者和RNA 免疫沉降测定以确认分子相互作用.
- 在体内瘤异种移植模型来评估功能角色.
主要成果:
- 在CircSLC25A16和CISD2上调,而在NSCLC中miR-335-5p下调.
- CircSLC25A16作为miR-335-5p的海绵,抑制其功能.
- CISD2是miR-335-5p的目标,而circSLC25A16对CISD2.2进行上调.
- 沉默circSLC25A16抑制了NSCLC细胞的增殖,迁移和糖解,并诱导了细胞亡,miR-335-5p抑制可以逆转这些效应.
结论:
- 循环SLC25A16通过miR-335-5p/CISD2路径驱动NSCLC的进展.
- CircSLC25A16显示出作为NSCLC的诊断生物标志物和治疗点的潜力.
相关概念视频
Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K


