四种野生丝状真菌为橄石生产的纤维细胞分解酶 价值化:比较三种发酵模式
Soukaina Arif1,2, Hasna Nait M'Barek2,3, Boris Bekaert4
1Moulay Ismail University of Meknès, Laboratory of Biotechnology and Bioresources Valorization, BP 11201, Zitoune Meknes City, Morocco.
Journal of microbiology and biotechnology
|May 28, 2024
概括
在橄石上培养了四种真菌,以产生纤维细胞分解酶. 虫 (Penicillium crustosum) 显示出高细胞酶活性,而虫 (Fusarium nygamai) 和虫 (Aspergillus calidoustus) 则显示出新的酶生产能力.
科学领域:
- 生物技术和工业微生物学
- 酶技术 酶技术是一种
- 生物质转换生物质转换
背景情况:
- 纤维解酶对于将纤维素转化为有价值的产品至关重要.
- 目前酶生产的局限性包括产量低,成本高,稳定性差.
- 利用本地,低成本的基质和适应的微生物生产者可以克服这些挑战.
研究的目的:
- 评估四种真菌物种 (Penicillium crustosum,Fusarium nygamai,Trichoderma capillare,Aspergillus calidoustus) 产生纤维细胞分解酶的潜力. 为了评估四种真菌物种 (Penicillium crustosum,Fusarium nygamai,Trichoderma capillare,Aspergillus calidoustus) 产生纤维细胞分解酶的潜力.
- 为了研究橄石 (OS) 作为酶生产的纤维素基质的有效性.
- 为了比较这些真菌在不同的发酵技术下 (浸泡发酵 - SmF,固态发酵 - SSF,序列发酵 - SF) 的酶生产能力.
主要方法:
- 在橄石上种植真菌,使用多层次的因数设计,在黑暗的化状态下长达15天.
- 对总细胞酶,β-葡萄糖酶,乳糖酶和内葡萄糖酶活动的测定.
- 确定蛋白质含量和最佳酶生产时间表.
主要成果:
- 在SF下,Penicillium crustosum达到最大的总细胞酶活性 (10.9 IU/ml),在SmF下达到最大的β-葡萄糖酶活性 (3.6 IU/ml).
- 在SSF下,Fusarium nygamai表现出最高的乳糖酶活性 (9IU/ml).
- Aspergillus calidoustus产生了显著的蛋白质产量 (1,009微克/毫升) 和内葡萄糖酶活性 (11.5 IU/毫升).
- 最佳的细胞酶产生发生在第5天 (SF),而线性酶在9-11天 (SSF) 之间达到峰值.
结论:
- 这项研究表明,使用橄石,P. crustosum,F. nygamai,T. capillare和A. calidoustus的纤维细胞分解酶产生潜力.
- 突出了F. nygamai和A. calidoustus的新型纤维素分解活性.
- 证实橄石是酶生产的有效诱导基质,支持环保的生物质分解应用.
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