鉴定circRNA表达特征和潜在的系统性免疫失衡调节在膜的过早破裂
Dongni Huang1,2, Yuxin Ran1,2, Ruixin Chen3
1Women and Children's Hospital of Chongqing Medical University (Chongqing Health Center for Women and Children), Chongqing, China.
Analytical cellular pathology (Amsterdam)
|May 28, 2024
概括
循环RNAs (circRNAs) 与膜过早破裂 (PROM) 有关. 这项研究揭示了circRNA介导的免疫失衡和原破坏作为PROM的潜在原因,提供了新的诊断和治疗途径.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 产科 产科 产科 产科 产科
背景情况:
- 膜早期破裂 (PROM) 是围产期发病率和死亡率的主要原因.
- 循环RNAs (circRNAs) 越来越多地被认为是各种疾病的关键调节者.
- 环RNAs在PROM中的作用和调节机制尚不清楚.
研究的目的:
- 为了研究PROM中的circRNA表达特征.
- 在PROM中阐明circRNA-miRNA-mRNA调节网络.
- 为了确定潜在的circRNA生物标记物和PROM的治疗点.
主要方法:
- 来自PROM和对照组的血和胎儿膜的CircRNA微阵列分析.
- 生物信息分析包括建立circRNA-miRNA-mRNA网络.
- 基因本体学 (GO),基因和基因组的京都百科全书 (KEGG) 途径和基因组丰富分析 (GSEA).
主要成果:
- 在PROM中鉴定出1,459个差异表达的circRNAs (DECs) (406个上调,1,053个下调).
- 构建了一个涉及22个circRNA-miRNA和128个miRNA-mRNA对的调节网络.
- DECs与免疫相关途径和细胞外基质调节有显著的关联,在胎儿膜和血中共有11个宿主基因.
结论:
- 循环RNAs可能会通过破坏系统和局部免疫平衡和原结构来使个体易患PROM.
- 这项研究提供了PROM中circRNAs的第一个全面景观.
- 这些发现通过基于circRNA的策略为PROM诊断和治疗开辟了新的途径.
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