新生儿败血症是由于NDM-1和VIM-2共同产生Pseudomonas aeruginosa在摩洛哥
Dina Daaboul1,2, Marwan Osman3, Issmat I Kassem4
1Team ReSIST, UMR1184, INSERM, Université Paris-Saclay, CEA, School of Medicine, OI HEALTHI, Le Kremlin-Bicêtre, France.
The Journal of antimicrobial chemotherapy
|May 28, 2024
概括
极其耐药的Pseudomonas aeruginosa在摩洛哥的一家NICU中共同表达了NDM-1和VIM-2碳烯酶. 这种高风险克隆 (ST773) 的治疗选择有限,突显了迫切的公共卫生问题.
科学领域:
- 医学微生物学 医学微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在全球范围内,抗卡巴因耐药性Pseudomonas aeruginosa的流行率正在上升.
- 需要了解极具耐药性 (XDR) 隔离物的耐药性机制.
研究的目的:
- 在摩洛哥新生儿重症监护室 (NICU) 的XDR P. aeruginosa分离物中研究碳烯胺耐药性的分子机制.
主要方法:
- 使用MALDI-TOF-MS.识别P. aeruginosa菌株O82J1的发现.
- 现型抗微生物敏感性测试 (Carba NP,NG-K5,磁盘扩散,微博).
- 整基因组测序 (Illumina和MinION) 可以预测抵抗体,序列类型和等离子体类型.
主要成果:
- 在P. aeruginosa O82J1中,金属β-乳酸酶 blaNDM-1 和 blaVIM-2 的共同表达.
- 菌株属于高风险克隆ST773;blaVIM-2是血传播的,blaNDM-1是染色体编码的.
- 检测到23个抗微生物药物耐药性基因,包括blaPER-1和rmtB,仅对胆固醇素和阿普拉胺素敏感.
结论:
- 在NICU中隔离XDR P. aeruginosa,由于有效抗生素的有限性,造成了重大治疗挑战.
- 对诸如 cefiderocol 和 ATM/AVI 这类新药的耐药性是治理格兰氏阴性感染的一个主要问题.
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