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Updated: Jun 25, 2025

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Published on: January 26, 2019

血管新生,矩阵重塑和抗菌因子在早产和长产的人类带的表征

Kaiva Zile Zarina¹, Mara Pilmane¹

¹Institute of Anatomy and Anthropology, Riga Stradins University, Kronvalda Boulevard 9, LV-1010 Riga, Latvia.

Journal of developmental biology

|May 28, 2024

概括

带成熟涉及矩阵重塑和原始细胞活动,矩阵金属蛋白酶-2 (MMP2) 和金属蛋白酶-2 (TIMP2) 的组织抑制剂发挥关键作用. 血管内皮生长因子 (VEGF) 和人类β-防御素2 (HBD2) 的表达一致,而CD34则表明早产线索中的祖细胞.

科学领域:

周围生理学周围生理学
发展生物学发展生物学
生物化学生化学

背景情况:

在成熟期间对带形态遗传变化的理解有限.
关键的过程,如细胞外矩阵重塑,血管新生,原生体活动,免疫调节等都涉及到.

研究的目的:

为了研究矩阵金属蛋白酶-2 (MMP2),金属蛋白酶-2 (TIMP2) 的组织抑制剂,CD34,血管内皮生长因子 (VEGF) 和人类β-防御素2 (HBD2) 的表达.
为了比较早产的标记物表达与全产的人类带组织.

主要方法:

来自17名患者的带组织样本被分为非常早产,中度早产,晚期早产和全产组.
免疫组织化学被用来检测标志物阳性细胞,通过显微镜进行半定量计数.
使用SPSS统计29进行了统计分析.

主要成果:

胚胎外介质细胞显示MMP2,TIMP2,VEGF和HBD2的高表达在早产和早产的绳索中.
在患者组之间观察到CD34,MMP2和TIMP2表达的显著差异.
在不同组中,VEGF表达是一致的,主要在胚外介质中;HBD2在胚外介质和胎内表皮中最高.

结论:

带中的细胞外矩阵重塑受MMP2和TIMP2的调节.
VEGF表达是独立于年龄的,但受个人因素的影响;CD34突出显示了早产线索中的祖细胞作用.
在早产和早产的带中,HBD2提供了必不可少的,持续的抗菌活性.

关键词:

CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 CD34 这就是HBD2 HBD2.这就是MMP2的MMP2.时间表2 TIMP2 在VEGF中,VEGF是VEGF.带是指带的一个部分.

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