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基于胆的表面活性离子液体的自组装和度依赖的增强细胞酶在水性介质中的酶活性
Manpreet Singh1, Gurbir Singh1, Harmandeep Kaur1
1Department of Chemistry, UGC-Centre for Advance Studies - II, Guru Nanak Dev University, Amritsar, 143005, India. tejwantsinghkang@gmail.com.
Physical chemistry chemical physics : PCCP
|May 28, 2024
概括
基于胆的阳离子表面活性剂形成微粒,显著增强细胞酶活性. 它们的化受离子水化和结合的影响,为基于酶的应用提供了潜力.
科学领域:
- 物理化学 物理化学
- 超分子化学 超分子化学
- 生物化学 生物化学
背景情况:
- 表面活性离子液体 (SAIL) 是可调节的两性化合物,具有多种应用.
- 基于胆的SAIL为传统表面活性剂提供了一个潜在的更绿色的替代品.
- 了解菌形成对于优化SAIL性能至关重要.
研究的目的:
- 研究基于胆的阳离子SAILs与不同反离子 ([Sar]-, [DS]-, [Doc]-) 的化行为.
- 探索这些SAILs对细胞酶活性的影响.
- 为了将细胞特性与观察到的酶增强相关联.
主要方法:
- 电子结构和相互作用的密度函数理论 (DFT).
- 表面张力,导电量和光光谱学用于关键菌度 (cmc) 和菌特性.
- 动态光散射 (DLS) 和小角度中子散射 (SANS) 用于微粒大小和形态.
- 异热定位热量测量 (ITC) 用于化的热力学参数.
主要成果:
- 帆船化是由胆头组,阴离子头组和反离子结合 (β) 的水合控制的.
- 与缓冲剂相比,单质SAIL溶液中的细胞酶活性显著增加 (7至13倍).
- 在微粒SAIL溶液中,酶活性进一步增强 (4至5倍),按照[Cho][Sar] > [Cho][DS] > [Cho][Doc]的顺序.
结论:
- 基于胆的阳离子SAIL表现出不同的化行为,受其离子成分的影响.
- 这些SAIL可以作为增强细胞酶活性的有效介质,其性能取决于度和反离子.
- 这些发现表明这些SAIL在生物催化剂和酶稳定中的潜在应用.
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