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在ProRoot MTA中氧化物的浸出和细胞毒性 - - 一个实验室调查

Lauter E Pelepenko¹, Marina A Marciano¹, Richard M Shelton²

¹Department of Restorative Dentistry, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, Brazil.

International endodontic journal

|May 28, 2024

概括

在180天内观察到ProRoot MTA中的石出,对细胞活力和基因表达产生负面影响. 这项研究是第一个表明ProRoot MTA影响金属氨酸基因表达的研究.

科学领域:

生物材料科学生物材料科学
牙科材料牙科材料
毒理学毒理学毒理学

背景情况:

矿物三氧化聚合物 (MTA) 在牙科中广泛使用.
氧化是MTA中常见的放射性平静剂,引发了人们对其潜在的浸出和细胞毒性的担忧.
了解石释放及其生物效应对于材料安全至关重要.

研究的目的:

为了调查从ProRoot MTA中水的水.
为了评估从ProRoot MTA释放的比斯木的细胞毒性.
为了确定水泥基层和浸泡条件是否会影响比斯木泄漏.

主要方法:

在180天内使用ICP-MS测量了ProRoot MTA中的石出.
细胞毒性通过使用Wistar大鼠骨髓 stromal 细胞 (BMSCs) 和纤维细胞被评估.
细胞活力和基因表达 (金属联胺,原-1a,骨蛋白) 在暴露于材料和浸出物后被分析.

主要成果:

在整个180天的研究中,从ProRoot MTA中检测到石水.
陈旧的ProRoot MTA显示出黑色变色和碳酸沉积.
暴露在ProRoot MTA及其180天的液中,降低了BMSC活力,并改变了金属氨酸,原-1a和骨蛋白基因表达.

结论:

来自ProRoot MTA的石液发生,并影响细胞活力和基因表达.
这项研究证明了ProRoot MTA对金属氨酸基因表达的影响.
这些发现强调了考虑木在牙科材料中的生物影响的重要性.

关键词:

这就是BMSCs.毕斯穆特 (bismuth) 是一种天然的纤维细胞的细胞.液酸盐是一种液酸盐.在金属乙中.

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