相关实验视频
Updated: Jun 25, 2025

05:07
Characterizing Extracellular Vesicles from Biological Fluids
Published on: February 28, 2025
300
表面涂层组成对细胞外囊分泌的细胞特异性影响
Yuan Liu1, Clifford J Pierre2, Sailesti Joshi1
1Department of Chemical & Biomedical Engineering, FAMU-FSU College of Engineering, Florida A&M University, Florida State University, 2525 Pottsdamer Street, Tallahasee, Florida 32310-6046, United States.
ACS applied materials & interfaces
|May 28, 2024
概括
生物材料表面涂层对各种细胞类型的细胞外囊泡 (EV) 生产和功能有不同的影响. 优化这些涂层是治疗EV应用的关键.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 细胞外囊泡生物学 细胞外囊泡生物学
背景情况:
- 生物材料的特性影响细胞外囊泡 (EV) 分泌和载荷.
- 基板成分对电动汽车生产的影响尚不清楚.
- 了解细胞外矩阵微环境对EVs的影响对于治疗应用至关重要.
研究的目的:
- 研究原I (COLI),纤维素 (FN) 和聚氨酸 (PLL) 表面涂层对EV分泌的影响.
- 描述细胞对不同基质涂层的特异反应.
- 评估从各种涂层上培养的细胞中获得的EVs的功能能力.
主要方法:
- 在COLI,FN和PLL涂层上培养的初级骨髓衍生中细胞 (BMSC),脂肪衍生干细胞 (ASC),HEK293细胞,NIH3T3细胞和RAW264.7细胞.
- 量化EV分泌和细胞活力.
- 分析了EV生物发生和细胞粘附基因的表达.
- 评估了衍生EVs对巨细胞增殖的体外功能能力.
主要成果:
- COLI涂层降低了RAW264.7细胞中的EV分泌,影响了细胞活力和基因表达.
- 在NIH3T3细胞中,FN涂层增加了EV分泌.
- PLL涂层增强了ASC中的EV分泌.
- 表面涂层在体外显著改变了电动汽车的功能能力.
结论:
- 表面涂层对EV分泌和功能能力产生细胞特异性影响.
- 基质组成是调节治疗目的EV生产的关键因素.
- 量身定制表面涂料有潜力推进临床电动汽车生产系统.
更多相关视频
相关概念视频
COP Coated Vesicles
7.8K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
7.8K
Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Clathrin Coated Vesicles
6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K

