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Updated: Jun 25, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

具有双层果卷主要囊蛋白质的真核DNA病毒的自然历史

Mart Krupovic¹, Jens H Kuhn², Matthias G Fischer³

¹Archaeal Virology Unit, Institut Pasteur, Université Paris Cité, Paris 75015, France.

Proceedings of the National Academy of Sciences of the United States of America

|May 28, 2024

概括

这项研究揭示了Preplasmiviricota病毒的进化历史,突出了它们的古老起源和所有细胞领域的多样化宿主相互作用. 关键的发现集中在蛋白质原始DNA聚合酶及其域,解释病毒进化.

科学领域:

病毒学病毒学
分子进化分子进化
结构生物学结构生物学

背景情况:

类Preplasmiviricota包括各种各样的病毒,具有小的双链DNA基因组和二元体囊.
这些病毒感染了所有细胞领域的宿主,包括与真核生物超群的显著关联,这表明它们的起源很古老.
预等离子病毒群包括波林顿,波林顿样病毒 (PLV),病毒细胞和腺病毒.

研究的目的:

为了阐明Preplasmiviricota类的进化起源和多样化.
分析这些病毒中的蛋白质原始DNA聚合酶 (pPolBs) 和相关域的结构和功能.
根据关键蛋白质域的存在,缺席和失活来重建进化途径.

主要方法:

蛋白质结构建模和比较分析.
对与终端蛋白 (TPs) 和病毒性卵巢瘤类半氨酸二维基提尼酶 (vOTU) 域同类的N终端域的研究.
对包括pPolBs在内的DNA聚合酶和各种病毒群中的超级家族1螺旋酶域的分析.

主要成果:

聚子,病毒细胞体和细胞质线性质粒中的pPolBs具有N端TP同源域和vOTU域.
vOTU域与TP裂变有关,其在腺病毒中无活化与TP作为单独的蛋白质相关.
许多PLV和转基因病毒表现出经过修改的pPolBs,保留TP和vOTU域,但被替换的外核酶域与超级家族1酶域.

结论:

这项研究概述了真核生物Preplasmiviricota的综合进化情景.
变化vOTU域状态和DNA聚合酶组成在塑造这些病毒系的进化方面至关重要.
这些发现提供了关于感染所有细胞生命的病毒的古代起源和多样化的见解.

关键词:

腺病毒的病毒.必须使用双基尼酶.波林顿斯波林顿斯波林顿斯波林顿斯蛋白质原料家族B-DNA聚合酶病毒细胞的病毒细胞.

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