结构,功能和EGFL7的重组生产
Brennan McDonald1, Mirko H H Schmidt1
19169 Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technische Universität Dresden School of Medicine, Fetscherstr. 74, D-01307 Dresden, Germany.
Biological chemistry
|May 28, 2024
概括
表皮生长因子类蛋白7 (EGFL7) 对于血管形成至关重要,并与癌症有关. 基于baculovirus的Sf9昆虫细胞系统是生产研究活性,稳定的重组EGFL7的最佳选择.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 皮肤外生长因子类蛋白7 (EGFL7) 是一种涉及血管生成,血管生成和神经生成的分泌因子.
- EGFL7在病理状况中发挥作用,例如人类癌症中的瘤血管生成.
- 了解EGFL7在血管形成中的作用对于治疗开发至关重要.
研究的目的:
- 审查生产复合EGFL7蛋白质的方法.
- 为了比较不同表达系统的优点和缺点,用于EGFL7生产.
主要方法:
- 关于EGFL7遗传学,结构和功能的概述.
- 对三种重组蛋白表达系统的检查:大肠杆菌 (E. coli),哺乳动物细胞 (HEK 293) 和基于baculovirus的Sf9昆虫细胞.
主要成果:
- 大肠杆菌,哺乳动物和昆虫细胞系统适合EGFL7生产的比较.
- 对每个系统的产量,蛋白质活性,形状和翻译后修改的评估.
结论:
- 基于baculovirus的Sf9昆虫细胞表达系统目前是生产活性重组EGFL7最有利的.
- 这种系统以原生形状的EGFL7产生,具有适当的翻译后修改,足够的产量和稳定性用于实验.
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