菌类和细菌在牙内占据着不同的空间
Rosalyn M Sulyanto1,2, Clifford J Beall3, Kasey Ha1,2
1Department of Dentistry, Boston Children's Hospital, Boston, Massachusetts, United States of America.
PLoS pathogens
|May 28, 2024
概括
菌类和细菌在牙内占据了单独的空间,很少共同殖民. 口腔微生物在牙中的这种独特的空间布局可能会影响牙虫的进展.
科学领域:
- 微生物学 微生物学
- 口腔生物学 口腔生物学
- 牙科研究 牙科研究
背景情况:
- 病病因主要归因于细菌,但真菌的作用越来越被认可.
- 先进的牙菌的微生物入侵需要进一步调查.
研究的目的:
- 为了评估真菌和细菌社区的组成和空间分布在各种牙科.
- 探索牙管内真菌和细菌之间的空间关系.
主要方法:
- 16S rRNA基因测序和定量PCR用于微生物分析.
- 组织学染色 (格洛科特银色,PAS,CW) 和免疫组织化学 (IHC).
- 场辐射扫描电子显微镜 (FESEM) 用于可视化微生物生物膜.
主要成果:
- 在牙相关的病变中,真菌与细菌的比率增加,Candida物种经常占主导地位.
- 组织学和IHC证实菌在牙管内存在.
- 菌类和细菌在牙管内占据了不同的空间,罕见的共同殖民.
结论:
- 展示了一种新的现象,即真菌和细菌在牙内占据了独立的空间.
- 口腔微生物的独特空间组织可能在牙腐烂的进展中发挥重要作用.
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