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通过破坏Drosophila中蛋白质完整性和表观遗传记忆的环境破坏的器官转化
Orli Snir1, Michael Elgart1, Yulia Gnainsky1
1Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
PLoS biology
|May 28, 2024
概括
环境因素可以通过破坏蛋白质完整性和表观遗传标记来重新编程整个器官. 这项研究揭示了如何在Drosophila中暴露于以太会通过改变的Hsp90和H3K4me3水平引起体到翅膀的转变.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 环境科学 环境科学
背景情况:
- 通过基因环境相互作用来理解器官重编程至关重要,但在机理上是模糊的.
- 细胞内表观遗传重编程得到了很好的研究,但整个器官水平的变化仍然不太了解.
研究的目的:
- 用环境触发器阐明器官重编程的机械基础.
- 为了研究胚胎以太暴露如何诱导Drosophila的杆到翅膀的转变.
主要方法:
- 作为一个模型系统,利用了Drosophila中以太诱导的到翅膀的转换.
- 分析了热冲击蛋白90 (Hsp90) 和Tritorax (trx) 在表观遗传修饰中的作用.
- 检查了H3K4me3染色体标记的变化及其对发育基因的影响.
主要成果:
- 以太暴露破坏了蛋白质的完整性,改变了Hsp90的部署,并抑制了Trithorax介导的H3K4me3.
- 特定的基因,如Ubx目标和翅膀发育基因,保持更高的H3K4me3水平,导致类似翅膀的结果.
- 降低Hsp90功能增强了转换的透性,并结合Hsp90和trx降低诱导没有以太的转换.
结论:
- 蛋白质完整性的环境破坏会影响组织组织组织基因甲基化和发育基因的表观遗传调节.
- 环境对Hsp90的"电容器功能"的减轻可以推动对祖先的身体计划的转变.
- 在表观遗传模式建立期间的陪伴反应可能提供了一个环境诱导器官转变的一般机制.
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