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在Fasciola hepatica的新芽细胞类干细胞
Paul McCusker1, Nathan G Clarke1, Erica Gardiner1
1Understanding Health & Disease, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.
PLoS pathogens
|May 28, 2024
概括
研究人员在普通肝 (Fasciola hepatica) 中发现了干细胞,称为新芽细胞. 这些细胞对于虫的发育至关重要,并且代表了新抗寄生虫药物的有希望的目标.
科学领域:
- 寄生虫学的寄生虫学
- 干细胞生物学 干细胞生物学
- 药物发现 药物发现 药物发现
背景情况:
- 由普通肝 (Fasciola hepatica) 引起的 Fasciolosis,导致农业食品行业的全球经济损失显著.
- 目前的药物对成人和青少年的突发症阶段都不够,需要确定新的药物点.
- 寄生虫平虫中的干细胞,称为新芽细胞,由于它们在发育和宿主-寄生虫相互作用中的作用,越来越多地被认为是潜在的目标.
研究的目的:
- 为了研究和描述负责Fasciola hepatica发育的增殖细胞.
- 为了确定这些细胞是否表现出其他平虫物种中发现的新芽细胞的特征.
- 探索这些增殖细胞作为新型甲虫药物点的潜力.
主要方法:
- 增殖细胞的自我更新,分化和辐射敏感性的表征.
- 在早期体外幼儿生长过程中对细胞增殖变化的分析.
- 经辐射诱导的新芽细胞切除后的虫的转录组分析.
- 通过RNA干扰介导的基因素H2B的淘汰,以评估其对新芽细胞样细胞和发育的影响.
主要成果:
- 在F. hepatica中,增殖细胞表现出与新芽细胞一致的属性,包括自我更新和分化能力.
- 在早期体外幼虫发育过程中观察到增殖细胞核面积的减少.
- 基于辐射的新芽细胞切除导致124个下调的转录,包括已知的干细胞标记物 (fgfrA,plk1),在Schistosoma mansoni中有68个同类.
- 核素H2B (一种增殖细胞标志物) 的敲除,切除了类似于新芽细胞的细胞,并损害了体外虫的发育.
结论:
- 在Fasciola hepatica中的增殖细胞在功能上相当于其他平虫物种中的新芽细胞.
- 这些类似神经细胞的细胞代表了开发新的抗虫治疗法西奥洛斯病的有吸引力的标.
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