使用并行采集-读取结构化照明显微镜 (PAR-SIM) 的超高时空分辨率成像
Xinzhu Xu1,2,3,4, Wenyi Wang1,3,5, Liang Qiao4,5
1Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, China.
Light, science & applications
|May 28, 2024
概括
并行获取-读取SIM (PAR-SIM) 提高了硬件层面的成像速度,达到132.9MPixels·s-1的速度. 这种超高分辨率技术可以捕捉到细胞动态,例如在408赫兹的线粒体融合,而不会增加相机成本.
科学领域:
- 生物物理学的生物物理.
- 光学显微镜的使用方法
- 细胞生物学 细胞生物学
背景情况:
- 结构化照明显微镜 (SIM) 是用于生物成像的超高分辨率技术.
- 目前增加SIM率的方法依赖于复杂的算法或昂贵的相机.
- 光毒性仍然是实时生物标本成像中的挑战.
研究的目的:
- 引入一个硬件层面的方法来提高SIM率和兴趣区域 (ROI) 覆盖率.
- 为了实现更高的成像速度,而无需额外的相机成本或复杂的算法.
- 为了证明新方法对活细胞成像的能力.
主要方法:
- 通过同步图案生成与图像曝光读取来开发并行获取-读取SIM (PAR-SIM).
- 利用探测器的全宽度来增强时空流动.
- 实现了132.9MPixels·s-1的时空流,分辨率为100nm.
主要成果:
- 与现有技术相比,PAR-SIM实现了9.6倍的时空流量增加.
- 在低信号条件下成功重建细胞器官,曝光时间超短.
- 在408赫兹的活COS-7细胞中捕获了线粒体动力学和膜融合.
结论:
- PAR-SIM通过一种新的并行曝光读出模式,显著提高了SIM的率.
- 这种方法为高速超高分辨率成像提供了具有成本效益的解决方案.
- 在其他先进的成像系统中,PAR-SIM战略有潜在的应用.
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