来自加密植物藻 Chroomonas placoidea 的PSII-ACPII二聚体的结构和明显的超分子组织
Zhiyuan Mao1,2, Xingyue Li1,2, Zhenhua Li1,2
1Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China.
研究人员揭示了加密藻类中超级复杂的光系统II-素甲/c结合蛋白 (PSII-ACPII) 的结构. 这一发现澄清了这些生态重要生物体中采光天线的独特布局.
科学领域:
- 光合作用研究研究光合作用.
- 结构生物学是结构生物学.
- 藻类生物化学 藻类生物化学
背景情况:
- 密码藻类是进化上不同的和生态上重要的光合作用真核生物.
- 这些藻类中的光系统II (PSII) 利用素甲和甲结合蛋白 (ACP) 来收集光,但它们的超分子组织仍然不清楚.
研究的目的:
- 确定加密植物藻 Chroomonas placoidea 中的 PSII-ACPII 超级复合物的结构组织.
- 为了阐明光采集天线蛋白在PSII核心周围的排列.
主要方法:
- 清除PSII-ACPII超级复合物中的Croomonas placoidea.
- 使用冷电子显微镜以2.47 Å分辨率分析超复杂结构.
主要成果:
- PSII-ACPII超级复合体采用了二维组织,其中两个PSII核心单体被六个对称排列的ACPII子单元所包围.
- 发现了一种新的Chl-a绑定天线子单元,CCPII-S,它调解了ACPII和PSII核心之间的相互作用.
- 与其他生物体中的PSII相比,ACPII子单位的组织呈现出明显的模式.
结论:
- 该研究提供了加密藻类中PSII-ACPII超级复合物的详细结构模型.
- 这些发现提供了关于线组装和加密藻类藻类光合作用中的潜在能量传输途径的见解.
- 结果突出了光合作用机械组织在不同藻类系的多样性.
更多相关视频
08:04A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
13:52Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
Published on: June 23, 2016
相关概念视频
Photosystems
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Photosystem I
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Photosystem II
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The Antenna Complex
The Photochemical Reaction Center
The Supercomplexes in the Crista Membrane
