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Circ_0001671通过miR-27b-3p/BLM轴调节前列腺癌的进展
Lihong Yang1, Yong Ruan2, Bin Chen1
1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Life Sciences, Guizhou University, Guiyang, 550025, China.
Scientific reports
|May 28, 2024
概括
循环RNA hsa_circ_0001671 (circ_0001671) 促进前列腺癌 (PCa) 的进展,通过海绵化miR-27b-3p和向上调节Bloom综合征蛋白 (BLM). 这表明circ_0001671是PCa.的潜在治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 前列腺癌 (PCa) 是男性的全球领先癌症,进展机制不明.
- 了解PCa的分子驱动因素对于开发有效治疗方法至关重要.
研究的目的:
- 为了研究hsa_circ_0001671 (circ_0001671) 在PCa病变发生过程中的作用.
- 阐明PCa细胞中circ_0001671的分子机制,重点关注竞争的内源RNA (ceRNA) 网络.
主要方法:
- 通过PCR,RNase R和桑格测序来识别circ_0001671.
- 使用生物信息学,光酶测定,FISH,RNA拉向下,和RIP验证circ_0001671/miR-27b-3p/BLM轴.
- 通过细胞增殖,迁移,入侵和细胞亡测定以及瘤生长研究,在体外和体内评估circ_0001671的功能.
- 基因表达分析通过qRT-PCR和西方布洛特.
主要成果:
- 在PCa组织和细胞系中,circ_0001671和Bloom综合征蛋白 (BLM) 的上调,而miR-27b-3p的下调.
- circ_0001671促进了PCa细胞的增殖,迁移和入侵,并抑制了细胞亡.
- 在体内研究证实circ_0001671在促进瘤生长中的作用.
- 机理学研究证实circ_0001671通过海绵化miR-27b-3p.p.作为BLM的ceRNA作用.
结论:
- circ_0001671 在前列腺癌中起着瘤基因的作用.
- circ_0001671的致癌作用通过miR-27b-3p/BLM轴进行介导.
- circ_0001671代表了PCa治疗的潜在新分子标.
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