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Updated: Jun 25, 2025

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
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用于多式成像工作流程的新鲜冷组织样本的通用准备通过形态和分子保存

Andreas Dannhorn1,2, Emine Kazanc2, Lucy Flint1

  • 1Imaging and Data analytics, Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Cambridge, UK.

Nature protocols
|May 28, 2024
PubMed
概括

一个新的工作流程保留了新鲜冷的组织形态和分子,用于多式成像. 这种快速的,通用的协议增强了组织分析,包括从人类和植物样本的质谱成像和空间奥米克.

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相关概念视频

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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