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在口腔状细胞癌中,FOXD2-AS1通过miR-378g/CRABP2轴促进恶性细胞行为
Shaoyong Guo1, Bixia Huang2, Zhisong You3
1Department of Stomatology, The First Hospital of Putian City, 449 Nanmen West Road, Chengxiang District, Putian City, Putian, 351100, China. 18650228266@163.com.
BMC oral health
|May 28, 2024
概括
长非编码RNAFOXD2-AS1通过调节竞争的内源RNA (ceRNA) 网络,促进口腔状细胞癌 (OSCC) 的进展. 这项研究揭示了FOXD2-AS1的存在.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 口腔状细胞癌 (OSCC) 是一种主要的全球性癌症,长非编码RNA (lncRNAs) 通过竞争的内源RNA (ceRNA) 网络参与其发展.
- 一种lncRNA的FOXD2-AS1在OSCC中具有已知的致癌作用,但其特定的ceRNA网络仍然未被描述.
研究的目的:
- 研究FOXD2-AS1在口腔状细胞癌 (OSCC) 进展中的作用.
- 阐明在OSCC中涉及FOXD2-AS1的竞争性内源RNA (ceRNA) 机制.
主要方法:
- 使用定量PCR和短毛RNA (shRNA) 转染来评估OSCC细胞中的FOXD2-AS1表达和功能.
- 功能丧失实验评估了FOXD2-AS1对细胞增殖,细胞亡,迁移和入侵的影响.
- RNA免疫沉降和光酶记者测定证实了FOXD2-AS1,miR-378g和CRABP2.2之间的相互作用.
主要成果:
- 在OSCC细胞系 (Cal27和SCC9) 中,FOXD2-AS1被显著上调.
- 沉默FOXD2-AS1抑制了OSCC细胞的增殖,迁移和入侵,同时增强了亡.
- 发现FOXD2-AS1与miR-378g相互作用,调节细胞视网膜酸结合蛋白2 (CRABP2) 表达,从而部分挽救了FOXD2-AS1耗尽的影响.
结论:
- 在口腔状细胞癌 (OSCC) 中,FOXD2-AS1促进恶性行为.
- FOXD2-AS1的致癌功能通过其与miR-378g的相互作用来调节ceRNA网络内的CRABP2表达.
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