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细胞外和应激反应途径是进化的油性Rhodotorula toruloides菌株多重应激耐受性的基础
Miguel Antunes1,2,3, Marta N Mota1,2,3, Isabel Sá-Correia4,5,6
1iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
Biotechnology for biofuels and bioproducts
|May 28, 2024
概括
适应性实验室进化 (ALE) 创造了一个强大的Rhodotorula toruloides菌株,提高了其对多种工业压力的耐受性. 这种工程酵母为改善微生物油和胡卜素生产提供了潜力.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 罗多托鲁拉 (Rhodotorula toruloides) 是一种红色的油性酵母酵母,可以从纤维素化物 (LCH) 中产生微生物油和胡卜素.
- 从IST536 (PYCC 5615) 开发出一种多重压力耐受菌株,在甲醇和高糖醇选择下使用适应性实验室进化 (ALE).
研究的目的:
- 为了研究基因组和转录基因组变化,在一个进化的R. toruloides菌株中研究多重压力耐受性的基础.
- 在工业酵母菌株改进中识别合成生物学应用的基因,途径和监管网络.
主要方法:
- 在选择性压力下的适应性实验室进化 (ALE).
- 基因组和转录基因组分析的比较.
- 多重压力耐受性的表型特征.
主要成果:
- 进化的菌株表现出减少的性,21,489个突变和242个副本数变异.
- 转录组分析揭示了634个不同表达的基因,特别是那些参与细胞表面和应激反应的基因.
- 这种菌株对透性,盐,乙醇,氧化,基因毒性和脂肪酸压力有增强的耐受性.
结论:
- 综合分析提供了对R. toruloides.多重压力耐受性获得的机制性见解.
- 确定了合成生物学有希望的目标,以提高工业酵母的强度.
- ALE是提高工业酵母菌株性能和耐受性的宝贵工具.
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